In the Gruneberg lab we are interested in understanding the different aspects that contribute to the faithfulness of chromosome segregation. Key questions we aim to address are:
- How is the formation of the mitotic spindle regulated?
- How do the chromosomes attach to the microtubules?
- How does the Spindle Assembly checkpoint work?
- How do reversible post-translational modifications such as phosphorylation and acetylation contribute to the coordination of cell cycle progression and chromosome segregation?
In order to study these processes we use mammalian tissue culture cells and employ a combination of biochemical and cell biological methods including imaging of both live and fixed cells as well as mass spectrometry and in vitro kinase and phosphatase assays.